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Cambridge enter the weekend in 16th position, which leaves them seven points clear of the relegation zone.
The U’s enter Saturday’s contest off the back of a 2-0 defeat to Charlton Athletic last weekend, which frustratingly ended a run of three consecutive victories in all competitions.
A Conor Washington brace was enough to condemn Cambridge to defeat in that fixture, and Mark Bonner will know that his side will be up against it to avoid a similar result when the league leaders come to town on Saturday.
A positive start will be needed if the hosts are to trouble Rotherham, but having failed to score in the opening 15 minutes at home in the league this season, it may be unlikely that Cambridge will fly out the traps.
Although, after losing once in their last eight home games in all competitions, Bonner may be hopeful that his side can frustrate the Millers at the weekend.
Despite suffering a defeat last weekend, Bonner is expected to name an unchanged team for Saturday.
That means James Brophy will keep his place in the side after coming into the starting lineup for the Charlton contest.
Joe Ironside will lead the line for the hosts, and Cambridge’s top scorer will look to add to the 11 league goals that he has scored in the league this term.
Rotherham are expected to welcome Wes Harding back into the starting lineup after the defender missed the midweek encounter with Burton Albion.
There will be no place in the squad for Mickel Miller, with a groin injury keeping the striker out of the fixture.
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With 19 goals between them, Michael Smith and Ladapo will continue their formidable forward partnership on Saturday.
Cambridge United possible starting lineup:
Mitov; Williams, Okedina, Iredale, Dunk; Weir, Digby, May; Smith, Brophy, Ironside
Rotherham United possible starting lineup:
Johansson; Harding, Edmonds-Green, Ihiekwe; Ogbene, Rathbone, Barlaser, Wiles, Ferguson; Ladapo, Smith