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Swansea have turned to Queens Park Rangers assistant boss Eustace after Steve Cooper left the Liberty Stadium earlier this week.
The Swans are due to play Forest Green Rovers in another friendly on Wednesday, 28 July.
A Swansea statement said: “After positive cases were identified, the club decided it was in the best interests of both players and staff – as well as everyone connected with Bristol Rovers – to cancel Saturday’s game in order to minimise the potential spread of the virus.
“In keeping with Welsh Government guidelines, those individuals who tested positive will now undergo a period of isolation.
“All staff and players within the first-team bubble will undergo ongoing monitoring and testing.
“All supporters who purchased a pass for the SwansTV Live stream will be refunded accordingly.
“We would like to thank Bristol Rovers for their understanding on this matter and wish them well for the season ahead.”