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The 35-year-old striker, who had not been involved in the Blades’ last three matches, has undergone surgery on a thigh injury sustained in training.
Sharp said in an Instagram post on Wednesday: “Always gutting to miss games so to be ruled out for the remainder of the season hurts.
“Sadly the injury was worse than I’d hoped but operation went well and will be back stronger next season to get my 250th goal in front of a packed Bramall Lane.”
Millwall enjoyed an impressive run in February, picking up three wins and two draws while suffering just one defeat.
They opened March with a hard-earned win over Preston North End, where Mason Bennett grabbed a late winner. Millwall then fell to a 2-0 home defeat against Blackburn Rovers the following week.
Gary Rowett’s men returned to winning ways away to struggling Derby County, grabbing a deserved win and piling miseries on the 19th-placed Rams.
Defender Shaun Hutchinson scored the only goal of the game in the 45th minute to give the visitors a 1-0 win over Wayne Rooney’s men.
However, Millwall were handed a second defeat in four games by Queens Park Rangers. QPR came from two goals down to grab a 3-2 win in the last game.